Enzyme-Linked Oligonucleotide Assay (ELONA).
Methods in molecular biology (Clifton, N.J.)(2023)
摘要
Aptamers are single-stranded oligonucleotides able to recognize a target with high affinity and specificity. Aptamers are used in different diagnostics applications, highlighting, among all, variations of the traditional enzyme-linked immunosorbent assay (ELISA). In this chapter, we show the procedures for the development of two types of indirect ELONA: a sandwich ELONA and a direct ELONA coupled to either real-time quantitative PCR as a direct and sensitive readout.
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关键词
Affinity characterization,Aptamers,ELONA,ELONA qPCR,SELEX
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