Multifactorial Evaluation of Regrouping Effects on Performance and Welfare in Two Italian Dual-Purpose Chicken Breeds: Bianca di Saluzzo and Bionda Piemontese

Simple Summary Local chicken breeds Bionda Piemontese (BP) and Bianca di Saluzzo (BS) are promising for use in small-scale poultry farms, which adopt free-range and/or organic farming practice. However, in practical conditions, it can happen that animals are subjected to regrouping, creating a new flock by mixing different groups. Such situations may provoke a stressful response in birds, compromising their welfare. To evaluate the resilience of BP and BS breeds to adverse management practices (regrouping), the present study aimed to assess the birds' welfare status through a multifactorial approach. As a result, the practice of regrouping immediately compromised the welfare status and the productive performances in both BP and BS hens, but, in the following weeks, birds displayed a reliance, which help them adapt to the new stressful conditions. The aim of the present study was to investigate the ability of two Italian slow-growing poultry breeds (namely, Bionda Piemontese, BP, and Bianca di Saluzzo, BS) to cope with a stressful event, such as collective grouping, using a multifactorial approach. A total of 120 hens of BP and BS breeds were homogenously distributed, according to breed, in 12 pens (10 hens/pen; 6 pens/breed), from 18 to 49 weeks of age. At 50 weeks of age, hens were regrouped (Stressful Farm Practice, SFP), by removing separators, both in indoor and outdoor areas. At 49 weeks of age, 24 hens/breed were randomly selected for the evaluation of welfare (ethological and physiological) parameters, at different time points: T0: 1-week pre-SPF; T1: 1-week post-SFP; T2: 3-week post-SFP; T3: 5-week post-SFP. Egg production was recorded from 38 to 56 weeks of age. Grouping produced a social stress in both BS and BP-laying hens, which was expressed in terms of productive traits (reduction of oviposition), behavioral modification (worsening of plumage condition due to feather peaking and extension of the duration of the tonic immobility test) and physiological modification (increased heterophil/lymphocyte ratio and corticosterone metabolites in droppings). Both breeds reacted in a similar way; in fact, no differences were attributed to the breed. At the end of the observation period, the egg rate fully recovered, while the behavioral and physiological parameters partially recovered but failed to recover to those recorded prior to the stressful event.