Highly efficient base editing in rabbit by using near-PAMless engineered CRISPR/Cas9 variants
Science China Life Sciences(2022)
摘要
Dear Editor,Base editors can achieve targeted C-to-T and A-to-G conversion without the generation of DNA double-strand breaks(DSBs) or the requirement of a donor template, showing the potential to generate new mutations or to correct pathogenic mutations (Gaudelli et al., 2018). However, for conventional base editors, efficient base editing requires the presence of an NGG protospacer adjacent motif (PAM) that lies 12–16 nt downstream of the target bases, which generally limits the application of these base editors (Gaudelli et al., 2018).
更多查看译文
关键词
efficient base editing,crispr/cas9 variants,rabbit,near-pamless
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要