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Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis

Bernadette C. Young,Stephen J. Bush,Sam Lipworth,Sophie George,Kate E. Dingle,Nick Sanderson,Alice Brankin,Timothy Walker,Srilakshmi Sharma, James Leong,Puneet Plaha,Monika Hofer,Peter Chiodini, B. Gottstein,Lavinia Furrer,Derrick Crook,Andrew Brent

OPEN FORUM INFECTIOUS DISEASES（2022）

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Abstract
Microbes unculturable in vitro remain diagnostically challenging, dependent historically on clinical findings, histology, or targeted molecular detection. We applied whole-genome sequencing directly from tissue to diagnose infections with mycobacteria (leprosy) and parasites (coenurosis). Direct pathogen DNA sequencing provides flexible solutions to diagnosis of difficult pathogens in diverse contexts.
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Key words
leprosy,coenurosis,Taenia,mycobacteria,whole genome sequencing
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