The Role of O-antigen in P1 Transduction of Shigella flexneri and Escherichia coli with its Alternative S' Tail Fibre

•P1 S' tail fibre allows transduction of phagemid DNA into E. coli O3, S. flexneri 2a 2457O and S. flexneri 5a M90T at a high efficiency.•Transduction of phagemid DNA by P1 with its S' tail fibre requires O-antigens of E. coli O3, S. flexneri 2a 2457O and S. flexneri 5a M90T.•LPS of E. coli O3, S. flexneri 2a 2457O and S. flexneri 5a M90T with O-antigens inhibit P1(S') transduction of phagemid DNA.•P1(S') transduction of phagemid DNA improved the Cas9 antimicrobial effect on S. flexneri 5a M90T and S. flexneri 2a 2457O.