Zinc finger structure determination by NMR: Why zinc fingers can be a handful

Progress in Nuclear Magnetic Resonance Spectroscopy（2022）

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摘要

•Overview of using NMR to determine solution structures of zinc finger proteins.•Practical and background information on sample preparation, stability and handling.•Methodology for including metals in structure calculations explicitly.•Determining metal-binding topology using 113Cd substitution and heteronuclear NMR.

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关键词

Zinc finger,NMR spectroscopy,NMR structure determination,Protein structure,Metallothionein

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