Green Method of CuO NPs by Using Eucalyptus Camaldulensis Aqueous Extract with Cold Plasma, and its effect on Biofilm Formation

The hot aqueous extract of E. camaldensis leaves as a degradable agent and copper nitrate as a copper source with cold plasma was used in this work to present an easy and green technique for copper oxide nanoparticles (CuO NPs) synthesis. UV-Vis absorption spectroscopy, X-ray diffraction (XRD), Atomic Force Microscopes (AFM), and Scanning Electron Microscopes (SEM) were all used to determine the production of copper oxide nanoparticles (SEM). The energy gap was 4.9 eV, and the average crystalline size of CuO NPs evaluated using the Scherrer equation was 15.06 nm, according to the X-ray diffraction pattern (XRD). CuO NPs had a grain size of 70.63 nanometers, according to AFM morphology study. Finally, the effect of copper oxide nanoparticles on Gram-positive and Gram-negative bacteria, as well as fungi, was studied.