On the hydrodynamic mutual interactions among cells for high-throughput microfluidic holographic cyto-tomography

•The dynamic of the rotation of adjacent cells in a tomographic flow cytometer is investigated with the aim to prevent mutual disturbing effects among them.•Both numerical simulation of the fluid dynamics and experimental data show that such hydrodynamic interactions are negligible, as they do not affect the possibility of recovering reliable tomograms.•The optimum density of cells can be estimated to allow the calculation of the maximum achievable throughput.