Extracellular expression of agarolytic enzymes in Clostridium sp. strain and its application for butanol production from Gelidium amansii
Bioresource Technology（2022）
Abstract
•Agarolytic genes of Aga0283 and NH2780 were over-expressed in strain WK-AN1.•Butanol production was improved from agar in the presence of glucose.•An optimal butanol production of 6.42 g/L was achieved from 17.86 g/L agar.•Conversion of G. amansii was established to achieve a maximum of 7.83 g/L butanol.
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Key words
Butanol,Agarase,Condition optimization,Gelidium amansii,Gene modification
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