米曲霉产米黑根毛霉脂肪酶高通量筛选方法的建立

XIONG Zhiyue, XU Dou,GUO Yuanxin,TIAN Xiwei,CHU Ju

Journal of East China University of Science and Technology(Natural Science Edition)（2022）

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Abstract

为了快速、高效地筛选出高产米黑根毛霉脂肪酶(RML)的米曲霉菌株,利用常温常压等离子体诱变技术(ARTP)对米曲霉孢子进行诱变,优化了高通量检测RML酶活的双指示剂法,建立了24孔板米曲霉高通量培养体系.通过对诱变后的1200孔(36000～48000个孢子)进行高通量筛选,最终筛选出4株米曲霉高产突变株.经由摇瓶验证,突变株Ⅰ7-D6-7、Ⅱ4-4B-5、Ⅲ3-5C-1、Ⅴ7-6C-3酶活分别达到了265.98、240.90、253.52、293.50 U/mL,比出发菌株的酶活(176.52 U/mL)分别提高了50.68％、36.47％、43.61％、66.27％.

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