Development of stable, cold-adapted, temperature-sensitive/conditional lethal chimeric enterovirus A71 and coxsackievirus A16.

•A stable EV-A71 virus vector was created to generate chimeric strains expressing capsid protein genes of EV-A71 C5 and CA16.•Phenotypic and genetic stability of the generated chimeric EV-A71 and CA16 were analyzed.•The amino acids at the cleavage site between VP1 and 2A is crucial for stability.