Temporary immersion bioreactor system for propagation by somatic embryogenesis of hybrid larch (Larix × eurolepis Henry)

Biotechnology Reports(2021)

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摘要
•First report demonstrating successful use of temporary-immersion bioreactors (TIBs) for the somatic embryogenesis process in the commercially important conifer hybrid larch Larix × eurolepis Henry•Two-fold increases in both multiplication (fresh weight of pro-embryogenic masses; PEMs) and yield of cotyledonary embryos in the TIBs compared to solid medium in plates•First report on germination of conifer somatic embryos in TIBs•Higher number of roots per plant, longer roots and taller plants in TIBs compared to germination on solid medium
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关键词
Germination,Horizontal TIB,Larix × eurolepis,Scale up,Somatic embryogenesis,Vertical TIB
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