Retention of organically bound deuterium in grass plants exposed to heavy water vapour at different growth stages

Potted timothy grass plants were exposed to heavy water (HDO) vapour at seven different periods during the third growth of harvestable shoots (the part 5-cm above the ground surface), and the concentration of non-exchangeable organically bound deuterium (NE-OBD) was measured in harvestable shoots, stubbles and roots at the third harvest. The excess concentration of NE-OBD was obtained as an increase in the concentration of NE-OBD against the background level. On average, this value was 1.8- to 2.6-fold higher in the harvestable shoots than in the stubble for plants exposed to HDO vapour during the period when the harvestable shoots grew faster than the stubble. Our results suggest that the separation of harvestable shoots and stubble, both of which are generally combined as one part in studies on the metabolism of organically bound tritium (OBT) in grass, could contribute to a realistic evaluation of OBT transfer from timothy to livestock.