Development and application of a High-Resolution mass spectrometry method for the detection of fentanyl analogs in urine and serum

•Utilization of the fentanyl analog screening kit from the Centers for Disease ControlDevelopment of a fentanyl analog high resolution mass spectrometry library.•Fentanyl analogs are rarely identified without the co-occurrence of fentanyl.•Fentanyl analogs fragmentation occurs at two specific bonds generating two dominate ions.