Validation of a simple and quick method for determination of psychoactive substances, drugs and their metabolites from human blood by LC-MS/MS

•Liquid-liquid extraction enables the rapid isolation of multiple analytes in one process.•LC-MS enables the simultaneous and unequivocal identification of a large number of analytes.•Rapid analysis of blood samples across a wide range of analytes allows the identification of toxic substances and rapid treatment initiation.•A quick, sensitive and specific method of analyzing a large number of psychoactive substances allows the detection of illegal substances.