Quantitative Detection of Mycobacterial Mannophosphoinositides in Tuberculosis Patients by Real-Time Immuno-Pcr Assay.
JOURNAL OF MICROBIOLOGICAL METHODS(2022)
摘要
A real-time immuno-PCR assay was deliberated to detect mycobacterial mannophosphoinositides (PIMs). A dynamic range of PIMs (0.9 pg/mL-10 ng/mL) was detected in TB patients, wherein 88.2% and 81.1% sensi-tivities were obtained in pulmonary TB and extrapulmonary TB respectively, with 96-96.4% specificity. This assay may translate into a diagnostic kit.
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关键词
Diagnosis,ELISA,Mtb,PIMs,Real-time immuno-PCR,TB
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