Quantitative Detection of Mycobacterial Mannophosphoinositides in Tuberculosis Patients by Real-Time Immuno-Pcr Assay.

Promod K. Mehta,Suman Sharma,Neeru Mehta,Bhawna Dahiya,Pamela Singh, Kavita Prashar,Abhishek Sheoran,Mandira Varma-Basil,Gopal K. Khuller

JOURNAL OF MICROBIOLOGICAL METHODS（2022）

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摘要

A real-time immuno-PCR assay was deliberated to detect mycobacterial mannophosphoinositides (PIMs). A dynamic range of PIMs (0.9 pg/mL-10 ng/mL) was detected in TB patients, wherein 88.2% and 81.1% sensi-tivities were obtained in pulmonary TB and extrapulmonary TB respectively, with 96-96.4% specificity. This assay may translate into a diagnostic kit.

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关键词

Diagnosis,ELISA,Mtb,PIMs,Real-time immuno-PCR,TB

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