Analysis of microrna biomarkers in inflammatory pathways regulation of pre-matured atherosclerosis in rats model

Background and Aims : MicroRNAs are highly up-regulated within atherosclerotic plaque complex indicating their heavy involvement in inflammatory genes regulation in atherosclerosis pathways. Hence, microRNA considered as a promising biomarker for the aforementioned disease. This study aims to investigate the changes in inflammatory microRNA expression in pre-matured atherosclerosis Sprague Dawley (SD) rats induced with high-fat diet.Methods: Rats (n=10) were randomly divided into High Fat Diet (HFD) and normal diet (ND) groups. Blood was collected for biochemistry analysis of lipid profiling, histology assessment of tunica intima on aorta by Haematoxylin and Eosin (H&E) staining and microRNAs analysis conducted in week 6 and week 12 using Real-Time PCR.Results: Biochemistry analysis shown significant correlation between HFD and ND and Low-Density Lipoprotein (LDL), High-Density Lipoprotein (HDL), Triglyceride (TG) and Total Cholesterol (TC) level from week 0 to 12, p<0.05. MicroRNA analysis revealed that, rno-miR-17-1-3p, rno-miR-210-5p, rno-miR-181a-2-3p and rno-miR-155-5p was expressed in both HFD and ND. There was significantly higher (p<0.05; t=11.80) expression of microRNAs in HFD group (27.15±8.90) compared to ND group (30.82±1.72). [z1] [z2] Microscopic analysis reveals the thickening on tunica intima of aorta due to inflammation activities in HFD (152.497±7.518 μm) compared to ND (148. 408±1.94 μm).Conclusions: High calorie diet influenced the expression pattern of the inflammatory microRNA biomarkers. The microRNA may play important role in the regulations of inflammatory gene in the development of pre-mature atherosclerosis. Background and Aims : MicroRNAs are highly up-regulated within atherosclerotic plaque complex indicating their heavy involvement in inflammatory genes regulation in atherosclerosis pathways. Hence, microRNA considered as a promising biomarker for the aforementioned disease. This study aims to investigate the changes in inflammatory microRNA expression in pre-matured atherosclerosis Sprague Dawley (SD) rats induced with high-fat diet. Methods: Rats (n=10) were randomly divided into High Fat Diet (HFD) and normal diet (ND) groups. Blood was collected for biochemistry analysis of lipid profiling, histology assessment of tunica intima on aorta by Haematoxylin and Eosin (H&E) staining and microRNAs analysis conducted in week 6 and week 12 using Real-Time PCR. Results: Biochemistry analysis shown significant correlation between HFD and ND and Low-Density Lipoprotein (LDL), High-Density Lipoprotein (HDL), Triglyceride (TG) and Total Cholesterol (TC) level from week 0 to 12, p<0.05. MicroRNA analysis revealed that, rno-miR-17-1-3p, rno-miR-210-5p, rno-miR-181a-2-3p and rno-miR-155-5p was expressed in both HFD and ND. There was significantly higher (p<0.05; t=11.80) expression of microRNAs in HFD group (27.15±8.90) compared to ND group (30.82±1.72). [z1] [z2] Microscopic analysis reveals the thickening on tunica intima of aorta due to inflammation activities in HFD (152.497±7.518 μm) compared to ND (148. 408±1.94 μm). Conclusions: High calorie diet influenced the expression pattern of the inflammatory microRNA biomarkers. The microRNA may play important role in the regulations of inflammatory gene in the development of pre-mature atherosclerosis.