Improved Sensitivity of NiO@R-Go Nanocomposite for Detecting H<sub>2</sub>S Biomaker of Halitosis Prognosis

A colorimetric approach for the quantitative detection of H2S based on a NiO@r-GO composite was developed in this study and is simple, quick, and low-cost. Bimetallic porous material NiO@r-GO with iodide adsorption into their framework have been shown to boost catalytic activity. In this study, we present a new method for enhancing NiO@r-GO peroxidase-like activity. Comparatively, the kinetic measurements of NiO@r-GO demonstrate that it has a great affinity for substrates, facilitating electron transport, as opposed to HRP. Improvements in the activity of NiO@r-GO are attributable to the synergistic effect of the two compounds and to the speedy electron transfer process. This method is very sensitive for H2S colorimetric technique with a LOD of 0.58 nM.