Paenibacillus arenilitoris sp. nov., isolated from seashore sand and genome mining revealed the biosynthesis potential as antibiotic producer

Strain IB182493 T , a marine, aerobic, Gram-stain-negative and motile bacterium, was isolated from seashore sand of South China Sea. Cells grew optimally at 25–30 °C, pH 7.0–8.0 and with 2–4% NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that the strain formed a distinct lineage within the genus Paenibacillus , and was most closely related to Paenibacillus harenae DSM 16969 T (similarity 96.6%) and Paenibacillus alkaliterrae DSM 17040 T (similarity 96.1%). The chemotaxonomic characteristics of strain IB182493 T included MK-7 as the predominant isoprenoid quinone, anteiso-C 15:0 and iso-C 16:0 as the major cellular fatty acids and meso -diaminopimelic acid as the diagnostic diaminoacid in cell wall peptidoglycan. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. The DNA G + C content of strain IB182493 T was 56.2 %. The values of whole genome average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) between the isolate and the closely related type strains were less than 84.7% and 23.6%, respectively. On the basis of phenotypic and chemotaxonomic properties, phylogenetic distinctiveness and genomic data, we named the strain as Paenibacillus arenilitoris sp. nov. and proposed that strain IB182493 T (= MCCC 1K04626 T = JCM 34215 T ) in the genus Paenibacillus represents a novel species.