铜藻中岩藻黄质提取及分离纯化工艺研究

CHEN Yaxin,HE Niaoniao,CAI Shuyun,YANG Ting,CHEN Hui,ZHANG Yi,HONG Zhuan,ZHANG Yiping

Science and Technology of Food Industry（2022）

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Abstract

优化铜藻中岩藻黄质提取工艺与分离纯化工艺.本研究以新鲜铜藻为原料,采用超声辅助有机溶剂萃取法提取,对岩藻黄质的提取工艺进行单因素实验与响应面法优化,随后采用6种不同大孔吸附树脂纯化岩藻黄质,进行静态吸附与解析动力学研究.结果表明:最佳提取工艺为:乙醇浓度95％,超声时间21 min,液料比38:1,提取次数为1次,所得岩藻黄质的提取得率为200.28μg/g(FW).拟一阶动力学模型能更好的诠释6种树脂吸附岩藻黄质的动力学行为,并结合动态吸附与解析结果筛选出最优性能的层析树脂为KP670型大孔吸附树脂(R2>0.99),一步柱层析使岩藻黄质质量分数从0.9％提高到31.07％.实验证明了该提取纯化铜藻中岩藻黄质的系列工艺是可行的.

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Key words

Sargassum horneri,fucoxanthin,response surface methodology,extraction,separation and purification,macroporous resin,kinetics

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