Fujian cytoplasmic male sterility and the fertility restorer gene OsRf19 provide a promising breeding system for hybrid rice.
Proceedings of the National Academy of Sciences of the United States of America(2022)
摘要
Cytoplasmic male sterility (CMS) determined by mitochondrial genes and restorer of fertility () controlled by nuclear-encoded genes provide the breeding systems of many hybrid crops for the utilization of heterosis. Although several CMS/ systems have been widely exploited in rice, hybrid breeding using these systems has encountered difficulties due to either fertility instability or complications of two-locus inheritance or both. In this work, we characterized a type of CMS, Fujian Abortive cytoplasmic male sterility (CMS-FA), with stable sporophytic male sterility and a nuclear restorer gene that completely restores hybrid fertility. CMS is caused by the chimeric open reading frame that specifically occurs in the mitochondrial genome of CMS-FA rice. The restorer gene encodes a pentatricopeptide repeat (PPR) protein targeted to mitochondria, where it mediates the cleavage of transcripts, thus restoring male fertility. Comparative sequence analysis revealed that originated through a recent duplication in wild rice relatives, sharing a common ancestor with /, a fertility restorer gene for Boro II and Hong-Lian CMS. We developed six restorer lines by introgressing into parental lines of elite CMS-WA hybrids; hybrids produced from these lines showed equivalent or better agronomic performance relative to their counterparts based on the CMS-WA system. These results demonstrate that CMS-FA/ provides a highly promising system for future hybrid rice breeding.
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关键词
OsRf19,cytoplasmic male sterility,hybrid rice breeding,restorer of fertility,rice
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