Saccharothrix obliqua sp. nov., isolated from soil of Sichang Island, Thailand

During an investigation of rare actinobacteria, isolate SC076(T) was isolated from a soil sample collected from Sichang Island, Chonburi Province, Thailand. The strain showed the highest 16S rRNA gene similarity to Saccharothrix australiensis DSM 43800(T) (98.6%) and Saccharothrix espanaensis DSM 44229(T) (98.6%). The zigzag morphology of the spore chain was observed on the aerial mycelia. meso-Diaminopimelic acid was detected in the peptidoglycan. Whole-cell sugars contained rhamnose, ribose, mannose glucose and galactose. Polar lipids were phosphatidylethanolamine, hydroxyphosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, unidentified ninhydrin-positive glycolipid, unidentified glycolipid and four unidentified lipids. The menaquinones were MK-9(H-8), MK-9(H-4), MK-9(H-2) and MK-9(H-0). The predominant fatty acids were iso-C-16:0, iso-C-15:0 and anteiso-C-17:0. The draft genome of SC076(T) was 8040245 bp with a G+C content of 72.5 mol%. The results of genomic analysis between strain SC076(T) and the related type strains showed that the digital DNA-DNA hybridization and average nucleotide identity values among the strains were 23.6-32.8% and 77.7-86.8 %, respectively, which are lower than the thresholds used to distinguish strains from others of the same species. Based on the taxonomic evidence, strain SC076(T) represents a novel species of the genus Saccharothrix for which the name Saccharothrix obliqua sp. nov. is proposed. The type strain is SC076(T) (=TBRC 14540(T)=NBRC 115117(T)).