Modified Ziehl–Neelsen staining for Mycobacterium leprae

Modified Ziehl–Neelsen staining for Mycobacterium leprae is a time-tested technique in the evaluation of leprosy. The staining helps to classify clinical types of leprosy into those with low and high bacillary load. It is also useful in assessing the response to therapy, identifying defaulters, and differentiating relapse from late reversal reactions. In this net educational video, we demonstrate the technique of taking an ear lobe smear and performing the modified Ziehl–Neelsen staining for acid fast bacilli.