Improving the thermostability of endo-β-1,4-glucanase by the fusion of a module subdivided from hyperthermophilic CBM9_1-2

Huabiao Miao,Yuanhao Zhao,Yu Ma,Nanyu Han,Yuanyuan Zhe,Xianghua Tang,Zunxi Huang

Process Biochemistry（2022）

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摘要

•Submodule (C2) was fused to the N- and/or C- termini of CMCase-I to produce variant glucanases.•The thermostability of the C2-CMCase-I chimera is 1.5 times greater than that of wild-type CMCase-I glucanase.•The chimera C2-CMCase-I forms a unique secondary structure.

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关键词

Endo-β-1,4-glucanase,CBM9_1-2,Fusion enzyme,Thermostability

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