The Effects of Camellia sinensis (Green Tea) Against Oxidized Helianthus annuus (Sunflower) Oil in Rabbits

The lipids oxidation is essential, keeping in view the taste, quality of nutritional components, and toxicity of lipids. We used oxidized sunflower oil combined with green tea to check its effects on hematological and serum biochemical parameters and liver in rabbits. Sunflower oil was oxidized continuously for 5 hours on hot plates at 100 degrees C. The rabbit groups were made, one group was fed a regular diet, and the other was fed with oxidized oil (2ml/kg body weight). Three groups were provided with green tea (50-50 ratio) at the dose rate of 100, 200, and 300 mg/kg combined with oxidized sunflower oil (2m/kg). Green tea alone was fed to two groups (200 mg and 100 mg/kg). The feeding regimen was continued for twenty-one days. The blood samples were collected on days zero, 11th, and 21st to analyze hematological and biochemical parameters. At the end of the experiment, one rabbit from each group was slaughtered, and the liver was extracted for histopathology. In the groups fed with oxidized sunflower oil alone or combined with green tea at various doses, significant alterations (P<0.05) in hematology and biochemical indices were observed. The high doses of green tea improved the alteration. No ill effect was observed in the groups fed green tea alone. Oxidized sunflower oil alone led to cholestasis and fatty changes in the liver, while green tea minimized the toxic effects confirming its anti-oxidant properties.