Substrate roughness influence on the order of nanografted Self-Assembled Monolayers

•The role of the substrate roughness in the packing order of the Self-Assembly Monolayers is clearly identify;•Phase imaging in Atomic and Electrostatic Force Microscopies enable to observed the order/disorder effect on Self-Assembly Monolayers;•The electrostatic force signal provides evidence that the conformational order versus the alkyl chain length follows an asymmetric parabolic trend induced by the substrate roughness;