Mn oxides enhanced pyrene removal with both rhizosphere and non-rhizosphere microorganisms in subsurface flow constructed wetlands.

The polycyclic aromatic hydrocarbons (PAHs) are substantial wastewater pollutants emitted mostly by petroleum refineries and petrochemical industries, and their environmental fate has been of increasing concern among the public. Consequently, subsurface flow constructed wetlands (SFCWs) filled with Mn oxides (W-CW) or without Mn oxides (K-CW) were established to investigate the performance and mechanisms of pyrene (PYR) removal. The average removal rates of PYR in W-CW and K-CW were 96.00% and 92.33%, respectively. The PYR removal via other pathways (microbial degradation, photolysis, volatilisation, etc.) occupied a sizeable proportion, while the total PYR content in K-CW plant roots was significantly higher than that of W-CW. The microorganisms on the root surface and rhizosphere played an important role in PYR degradation in W-CW and K-CW and were higher in W-CW than that in K-CW in all matrix zones. The microorganisms between the 10-16 cm zone from the bottom of W-CW filled with Mn oxides (W-16) were positively correlated with PYR-degrading microorganisms, aerobic bacteria and facultative anaerobes, whereas K-16 without birnessite-coated sand was negatively correlated with these microorganisms.