Development and application of a recombinant Envelope Domain III protein based indirect human IgM ELISA for Kyasanur forest disease virus

Aradhana Rajak,Jyoti S Kumar,Suman Dhankher, V K Sandhya,S K Kiran,Ramarao Golime,Paban Kumar Dash

Acta Tropica（2022）

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摘要

•KFD is an emerging tick-borne viral disease of public health significance.•Current serological assays need BSL-4 lab for antigen production.•Prokaryotic system used to express a safe highly immunogenic rEDIII protein.•Indirect ELISA system for detection of human anti KFDV IgM antibodies developed.•The first report on novel recombinant antigen-based ELISA for KFD infection.

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关键词

KFD,Diagnosis,IgM,Recombinant protein,Envelope Domain III,ELISA

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