The use of femtosecond laser pulses for controlled laser-assisted hatching of fresh and frozen/thawed mammalian embryos
2022 International Conference Laser Optics (ICLO)(2022)
摘要
Femtosecond laser pulses were applied for the microsurgery of mammalian embryos at the late stages of preimplantation development. An artificial opening in the outer envelope of mouse blastocysts was created and promoted hatching to start immediately through the hole formed. Laser exposure parameters were optimized to perform delicate laser-assisted hatching either on fresh or frozen/thawed embryos.
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关键词
frozen/thawed mammalian embryos,femtosecond laser pulses,laser-assisted
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