Evaluation of novel L-histidine-based Schiff base derivatives as microbial-HO inhibitors and their antimicrobial and molecular docking studies

•Efficient L-Histidine-based Schiff base derivatives (HKA-1,2 and 3) were synthesized and characterized by spectroscopic techniques like FTIR, 1H NMR, 13C NMR, HRMS, and circular dichroism.•The compounds had potential Microbial-HO enzyme inhibitory activity and were confirmed to be non-competitive inhibitors of microbial-HO's by Lineweaver-Burk plot.•The compounds were found to have antimicrobial activity against the microorganisms tested.•The compounds were confirmed to be non-haemolytic in human erythrocyte model.•The molecular docking studies revealed good G-Score values that support enzyme inhibition study.