Microwave-Assisted Synthesis, Characterization, Docking Studies and Molecular Dynamic of Some Novel Phenyl Thiazole Analogs as Xanthine Oxidase Inhibitor

A series of novel substituted 2-(4-bromophenyl)-4-phenylthiazole compounds 5a–h were synthesized by a simplified two-step process. First, the selective and effective α-bromination of substituted acetophenone moieties with N-bromo succinamide was done followed by treating of substituted 2-bromo-1-phenylethan-1-one with 4-bromobenzothiamide under microwave irradiation. The structures of the newly synthesized compounds were confirmed by IR, 1 H NMR, 13 C NMR, mass spectra and C, H, N analysis. Further, all the synthesized compounds 5a–h were evaluated for xanthine oxidase inhibition. Among all the tested compounds, 5f was found to be highly potent (IC 50 = 0.100 ± 0.08 µM) followed by 5e (IC 50 = 0.145 ± 1.42 µM), compared to the standard allopurinol (IC 50 = 0.150 ± 0.07 µM), which is evident from in vitro and in silico analysis.