EVALUATION OF ANTI QUOROM SENSING ACTIVITIES USING Emblica officinalis ETHANOLIC LEAF EXTRACTS AGAINST MARINE MICROBES

Biofilm formation in pathogenic bacteria displays a major role in various human infectious diseases posing a major health problem in several countries. Biofilms contain colony of bacteria which are 1,000 folds more resistant to antibiotics and also they develop more insensitivity to innate and acquired immune responses. Quorum sensing (QS) is characterized well due to the interaction of bacterial cells, which is a dependent mechanism and acts its stimulus response related to population density. In the present study we tested the antiquorum sensing activity with leaf extracts of commonly used dietary supplement Emblica Officinalis (Amla) against marine microbes. In our method, we cultured Klebsilla, Proteus, E.coli, Shigella, Salmonella typhi from marine water source for quorum sensing activities and inhibitory effects through antimicrobial activities. Marine bacteria were isolated, identified based on physical and biochemical characterization. In our results we investigated the inhibitory effects of amla against pathogenic bacteria using swarming method. Further highest inhibitory bacterial isolates were examined under scanning electron microscopy (SEM). We conclude that amla exhibits potential inhibitory effects in biofilms formation from samples obtained from marine waters and further studies are needed in QS gene expression in more number of marine microbial samples.