Immobilizing diamine oxidase on electroactive phase-change microcapsules to construct thermoregulatory smart biosensor for enhancing detection of histamine in foods
Food Chemistry(2022)
摘要
Aiming at enhancing the biosensing detection of histamine in foods at high temperature, we developed a ther-moregulatory biosensor based on diamine oxidase-immobilized phase-change microcapsules consisting of an n- docosane core, a TiO2 shell, and an electroactive polyaniline/ZnO composite coating layer. The microcapsules exhibit a satisfactory latent heat capacity of over 112 J/g for thermo-temperature regulation. Through an innovative integration of electroactive phase-change microcapsules and biological enzyme in the working electrode, the biosensor obtained a thermoregulatory function through reversible phase transitions by the n- docosane core under high-temperature environments. This enables the biosensor to achieve a higher response sensitivity of 28.57 mu A.mM(-1).cm(-2) and a lower detection limit of 0.473 mu mol/L at the high assay temperatures compared to conventional histamine biosensors. With enhanced electrochemical biosensing performance through in-situ thermo-temperature regulation, the smart biosensor developed in this study has found practical applications for high-sensitive detection and high-accurate quantitive determination of histamine in foods across a broad temperature range.
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关键词
Thermoregulatory smart biosensor,Electroactive phase-change microcapsules,Diamine oxidase,Electrochemical response,Biosensing performance,Histamine detection
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