ANTIMICROBIAL AND PHYTOCHEMICAL SCREENING OF EXTRACTS PRODUCED BY ENDOPHYTIC FUNGI ISOLATED FROM THE ROOT OF Lawsonia inermis ANTIMICROBIAL AND PHYTOCHEMICAL SCREENING OF EXTRACTS PRODUCED BY ENDOPHYTIC FUNGI ISOLATED FROM THE ROOT OF Lawsonia inermis

Endophytic fungi produced a secondary metabolite similar to their host plant; therefore it can be used as a source of producing active metabolites which lead in drug discoveries. The present study evaluate the antimicrobial activity of endophytic fungi from Lawsonia inermis and isolation of bioactive compounds for new antimicrobial agent. Fresh plant root materials were used for the isolation process, Aspergillus niger was identified as the species present on the plant. The screening of fungal for biological active secondary metabolites was carried out on malt broth fermentation. The broth filtrate was extracted with ethyl acetate and partitioned using a separating funnel. Disc diffusion methods of an antimicrobial sensitivity test at 100μg/disc, 50μg/disc, 25μg/disc 12.5μg/disc was adopted in testing the bioactivity of the extract against two clinical bacterial isolates of staphylococcus Aureus, Escheria coli and two clinical fungal isolates of Candida Albican and Aspergillus Flavus using disc diffution techniques. The extract were further subjected to phytochemical screening to detect the presence of secondary metabolites using standard procedures. The sensitivity test results showed that all the tested isolates are sensitive to extract at 12.5µg/disc as follows: Staphylococcus Aureus (10mm) , Escheria coli (11mm) , Candida Albican (7mm) and Aspergillus Flavus (8mm) . The result of phytochemical screening indicates the presence of alkaloids, cardical glycosides ,resins , saponins ,tannins , triterpenoids .The bioassay from the ethyl acecate fraction (HR) of the metabolite using column chromatography ,lead to the isolation of three pure compounds. The results of the antimicrobial activity of the isolated compounds indicated that (HR-102) was more potent against candida albican (11mm) at 12.5μg/disc followed by staphylococcus aureus (10mm) at 12.5μg/disc, escheria coli (9mm) at 12.5μg/disc, aspergillus flavus (8mm) at 12.5μg/disc. This signifies the potency for the development of therapeutic agent against pathogenic microorganisms. phytochemical screening of the three extracts fraction revealed the presence of alkaloids , steroids ,resins , triterpenoids .