Fast Single-Layer Reconstruction for Three-Dimensional Structured Illumination Microscopy

Ming Lei,ZhaoJun Wang,Tianyu Zhao,Yansheng Liang,Shaowei Wang,Yanan Cai,Jingxiang Zhang,Tongsheng Chen

SSRN Electronic Journal（2022）

Cited 1|Views23

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Abstract

•Single-layer three-dimensional structured illumination microscope (SL3D-SIM) can achieve fast continuous imaging of living cells at certain depth.•The reconstruction algorithms for SL3D-SIM were mostly constructed on the Wiener-SIM framework which is complex and time-consuming.•Joint space-frequency reconstruction (JSFR) framework establishes a fast reconstruction method for SL3D-SIM.•A 90 times improvement of the reconstruction speed to that of the conventional method.

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Key words

Single-layer reconstruction,Three-dimensional super-resolution microscopy,Structured illumination microscopy,Real-time imaging,Living-cell imaging

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