Comprehensive study of pydiflumetofen in Danio rerio: Enantioselective insight into the toxic mechanism and fate

•The toxic effects of R-PYD against zebrafish was higher than S-enantiomer.•Stronger binding of R with SDH causing lower enzyme activity led to higher toxicity.•Enantioselectivity of uptake, distribution and metabolism of PYD was observed.•The phase I and phase II metabolic pathways of PYD in zebrafish were first proposed.•Several highly toxic metabolites need to be given more attention in the future.