肾虚质、脾虚质大鼠唾液腺TNF-α、IFN-γ、IL-10表达差异性研究
China Journal of Chinese Medicine(2022)
Abstract
目的:基于"肾-唾、脾-涎"理论,考察肾虚质、脾虚质大鼠唾液腺肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、γ-干扰素(interferon-γ,IFN-γ)、白细胞介素-10(interleukin-10,IL-10)的表达差异.方法:采用"猫吓鼠"造模法制备先天不足+后天失养复合型肾虚质模型,采用饮食不节+劳倦过度法制备脾虚质模型,正常孕鼠所产仔鼠为平和质组.模型建立完成后,比较各组仔鼠的体质量;收集唾液,检测各组仔鼠唾液pH值;ELISA法检测唾液腺中TNF-α、IFN-γ、IL-10的含量.结果:与平和质组比较,肾虚质组仔鼠第8周体质量显著降低(P<0.01),脾虚质组仔鼠第6、8周体质量均显著降低(P<0.01);肾虚质组、脾虚质组仔鼠唾液pH值显著升高(P<0.01),唾液腺中IFN-γ、IL-10的含量显著降低(P<0.01);脾虚质组仔鼠唾液腺中TNF-α含量显著升高(P<0.05).与肾虚质组比较,脾虚质组第6、8周时仔鼠体质量均显著降低(P<0.01),但唾液pH值及唾液腺中TNF-α、IFN-γ、IL-10含量无明显变化.结论:肾虚质、脾虚质组大鼠免疫稳态失衡,但二者组间差异不明显,提示唾液为脾肾共同所主.
MoreAI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined