Triterpene Tetraglycosides From Stichopus Herrmanni Semper, 1868

Using various column chromatographic methods, 5 triterpene tetraglycosides (1-5), including one new compound, namely holothurin A(6) (1), were obtained from the water soluble part of the methanol extract of the sea cucumber Stichopus herrmanni. Their structures were confirmed by careful analysis of the 1D and 2D NMR, and HR ESI QTOF mass spectra. Noteworthily, 24-dehydroechinoside A (3) showed potent cytotoxicity to 5 human cancer cell lines {HepG2 (hepatoma cancer), KB (epidermoid carcinoma), LNCaP (prostate cancer), MCF7 (breast cancer), and SK-Mel2 (melanoma)} with IC50 values ranging from 0.19 +/- 0.03 to 1.17 +/- 0.18 mu M.