Two new azaphilones from the marine-derived fungus Penicillium sclerotiorum E23Y-1A

Two new azaphilones, penicilazaphilones F (1) and G (2) together with two known analogs (3 and 4), were isolated from fermentation cultures of the marine-derived fungus Penicillium sclerotiorum E23Y-1A. Their structures were elucidated based on spectroscopic methods, including IR, MS, 1D and 2D NMR, as well as by comparison with published data. The absolute configuration of compound 1 was unambiguously determined using electronic circular dichroism (ECD) calculation. All isolated compounds were evaluated for their anti-inflammatory effects. Compounds 1-4 inhibited the lipopolysaccharide-induced production of nitric oxide (NO) in BV-2 cells, with IC50 values of 31.7 +/- 1.5, 34.5 +/- 1.4, 25.3 +/- 2.2, and 34.8 +/- 1.9 mu M, respectively. In contrast, these compounds showed no obvious cytotoxicity at a concentration of 50.0 mu M.