MICRO-RAMAN SPECTROSCOPY CHARACTERIZATION OF MAGNETO-PRIMED AND DROUGHT-STRESSED TRITICALE SEEDS

Priming techniques such as magneto-priming are being broadly used in several studies focused on enhancing seed germination. Simultaneously, researchers have been performing tests on many species using PEG and NaCl, which generate osmotic stress conditions, proving that germination parameters are considerably delayed. The microscopic structure of seeds can be investigated using the Micro-Raman spectroscopic technique. The effects of magneto-priming and osmotic stress on the Triticale seed germination were studied with regards to the compounds identified in the pericarp, germ and endosperm using Micro-Raman. It was proved that Micro-Raman with magneto-priming together is a convenient approach to obtain and analyse information from the Triticale seed key components, mainly the ones in the endosperm. Although the response of the pericarp to this technique was feeble, generally the spectra ensued from the germ were rewarding. The identification of chemical compounds indicates that applying magnetism to seeds make them germinate earlier under stress conditions.