Identification of a Wheat Powdery Mildew Dominant Resistance Gene in the Pm5 Locus for High-Throughput Marker-Assisted Selection.

Powdery mildew, caused by f. sp. (), poses a severe threat to wheat yield and quality worldwide. Rapid identification and the accurate transference of effective resistance genes are important to the development of resistant cultivars and the sustainable control of this disease. In the present study, the wheat line AL11 exhibited high levels of resistance to powdery mildew at both the seedling and adult plant stages. Genetic analysis of the AL11 × 'Shixin 733' mapping population revealed that its resistance was controlled by a single dominant gene, tentatively designated . Using bulked segregant RNA-Seq and molecular marker analysis, was mapped to the locus on chromosome 7B where it cosegregated with the functional marker . Sequence alignment analysis revealed that the -homologous sequence in AL11 was identical to the reported recessive gene in wheat landrace 'Fuzhuang 30'. It appears that was most probably , but it was mediated by a dominant inheritance pattern, so it should provide a valuable resistance resource for both genetic study and wheat breeding. To efficiently use and trace in breeding, a new kompetitive allele-specific PCR marker that cosegregated with this gene was developed and confirmed to be applicable in the different wheat backgrounds, thus promoting its use in the marker-assisted selection of .