Liquid chromatography method for simultaneous quantification of ATP and its degradation products compatible with both UV–Vis and mass spectrometry

•Baseline separation of ATP and its 9 degradation products with reverse phase UPLC.•Volatile buffers allow simultaneous detection by UV–Vis and MS.•Phosphate acid wash and iron chelator are required to limit peak tailing.•Applicable to complex matrix of contracted skeletal muscle extracts.