应用MALDI-TOF-MS快速鉴定从鸡胴体中分离的一株空肠弯曲菌
Industrial Microbiology(2022)
Abstract
为快速鉴定从鸡胴体中分离的一株革兰氏阴性菌,应用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time of-flight mass spectrometry,MALDI-TOF-MS)技术对其进行鉴定.采用细菌分离纯化、MALDI-TOF-MS法鉴定,并以VITEK传统生化验证MALDI-TOF-MS鉴定结果的准确性.应用MALDI-TOF-MS法鉴定的空肠弯曲菌,鉴定分数为2.538,鉴定结果与VITEK一致.MALDI-TOF-MS方法能够快速、准确鉴定分离的空肠弯曲菌,为空肠弯曲菌的快速检测提供技术支撑.
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