Abeta-induced presynaptic release of UBC9 through extracellular vesicles involves SNAP23

•Aβ induces the redistribution of UBC9 from presynaptic to postsynaptic terminals.•Aβ mediates presynaptic release of UBC9 by extracellular vesicles.•SNAP-23 is involved in extracellular vesicles-mediated presynaptic release of UBC9.•Phosphorylation of SNAP-23 promoted Aβ-induced presynaptic release of UBC9.•The study reveals the mechanism of extracellular vesicles-mediated presynaptic release of UBC9.