Effect of FOXN2 Overexpression on Glioblastoma

Objective: This study was aimed to investigate the expression of FOXN2 in different grades of human gliomas and its effects on the proliferation, migration and invasion of human glioblastoma cells. Methods: The expression of FOXN2 in human glioma tissues and cells was detected by real-time quantitative polymerase chain reaction (qRT-PCR), Western Blot and immunofluorescence. The relationship between FOXN2 expression and survival time of patients was analyzed by CGGA (Chinese Glioma Genome Atlas) database [1]. Finally, after the lentivirus overexpressing FOXN2, we detected the effect of FOXN2 on glioma by MTT, Scratch wound healing, cell plate cloning assays, Transwell invasion assays and subcutaneous tumorigenesis assays in nude mice to determine the effect of FOXN2 on GBM. Results: We found that FOXN2 was low expressed in glioma tissues and cells, and its expression was negatively correlated with thegrade of glioma.The CGGA database showed that the higher the expression of FOXN2, the longer the survival time of patients. Fascinating, overexpression of FOXN2 significantly inhibited glioblastoma proliferation, invasion and migration, while it suppressed the growth and promoted tumor apoptosis of subcutaneously xenografts tumors in nude mice. Conclusion: FOXN2 acts as a tumor suppressor in gliomas and is likely to be a new potential target and prognostic biomarker for glioma.Funding Information: This work was supported by the Funding of “Peak” Training Program for Scientific Research of Yijishan Hospital (PF2019003)and the Natural Science Foundation of Anhui Province (6KJ2014A262). Declaration of Interests: There was no conflict of interest. We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product and service. Ethics Approval Statement: This protocol of the present study was approved by the Ethics Committee of Yijishan Hospital.