Ab1246 analysis of 38 cytokines profile in children with systemic lupus erythematosus

Backgroundsystemic lupus erythematosus (SLE) as “the disease of thousand faces” has multiple pathogenic mechanisms with disturbance in different parts of immune system. A variety of cytokines have been found as a biomarker of active disease and a candidate for targeting treatment [1].Objectivesto analyze 38 cytokines profile in juvenile SLE (jSLE) and compare them with disease activity and clinical manifestations.Methods29 patients with SLE under 18 years (3 boys, 26 girls, median age – 16.0 (13.0; 16.9) and 28 health control patients (14 boys, 14 girls, median age 16.0 (11.0; 17.0) were included in the study. Cytokine levels in the serum were measured by multiplex analysis using the MILLIPLEX MAP Human Cytokine/Chemokine/Growth Factor Panel A 41 plex kit (MilliporeSigma, USA) on the Luminex MAGPIX System (Luminex, USA).Resultssignificant differences between jSLE patients and health control have observed in 10 cytokines (Table 1). Interestingly upregulation in jSLE compared to HC was found only in IP10 and IL1RA, while other cytokines were lower. MCP1 was associated with disease activity measured by SLEDAI (p=0.0073), while activity measured by ECLAM correlated with TGFa (p=0.049), MCP3 (p=0.025), IL-15 (0.038), IL-7 (p=0.002), IL-8 (0.043), MCP1 (p=0.014), MIP1b (p=0.014). Positive correlation was found between skin involvement and IL-6 (p=0.43), carditis and FLT-3L (p=0.018), IL1RA (p=0.03), IL-8 (p=0.019), TNFa (p=0.05), lung involvement and FLT3-3L (p=0.014), IL-15 (p=0.047), IL1RA (p=0.034), IL-5 (p=0.005), TNFa (p=0.004), myositis and TGFa (p=0.011), MCP3 (p=0.000), IL-13 (p=0.000), IL1a (p=0.000), IL-9 (p=0.000), IL1b (p=0.03), IL-4 (p=0.000) TNFb (p=0.000), anti-dsDNA levels and MDC (p=0.016), anemia and GRO (p=0.030), IL-1b (p=0.050), IL-3 (p=0.022). There’s no correlation between nephritis, CNS involvement and cytokines levels.Table 1.CytokinesjSLE (n=29)HC (n=28)p-valueEGF28.4 (15.3-133.3)122.0 (25.8-218.5)0.032TGFa1.4 (0.0-2.6)4.6 (3.2-7.7)0.00001IL-103.8 (2.8-8.7)2.4 (2.0-3.6)0.034MDC461.3 (297.8-663.3)1042.5 (808.9-1277.5)0.00000sCD40L945.6 (126.5-1322.0)1991.5 (1275.0-2395.0)0.00003IL1RA21.9 (12.3-38.7)12.3 (8.6-19.4)0.013IL1b0.9 (0.6-1.2)1.0 (0.9-1.9)0.010IL417.3 (11.1-32.2)35.2 (19.6-87.8)0.003IL51.0 (0.8-1.4)1.6 (1.0-3.0)0.038IL89.1 (7.4-21.3)30.1 (11.5-46.5)0.010IP10310.9 (215.6-472.1)118.0 (89.3-143.7)0.000009Conclusionwe identified differences in cytokines levels between jSLE patients and HC and found multiple correlation between manifestations and different cytokines levels. Most of them were in FLT-3L, MIP-1b, TNFa, IL1RA, IL-3, IL-5, IL-8, IL-15, MCP1 and MCP3.References[1]Idborg H, Oke V. Cytokines as Biomarkers in Systemic Lupus Erythematosus: Value for Diagnosis and Drug Therapy. Int J Mol Sci. 2021;22(21):11327. Published 2021 Oct 20. doi:10.3390/ijms222111327AcknowledgementsThis research was funded by the Ministry of Science and Higher Education of the Russian Federation (Agreement No. 075-15-2020-901)Disclosure of InterestsNone declared