Antischistosomal Activity of Trigonella foenum (Fenugreek) Crude Seeds Water Extract on Infected Mice

Anti-parasitic activity of the fenugreek crude seeds water extract (FWE) on Schistosoma mansoni infected mice was studied in the present work and Praziquantel (PZQ) was applied as a standard drug.FWE was chemically analyzed by the high-performance liquid chromatography (HPLC) technique.The main recorded constituents were rutin, P-coumaric, hydroxyl-benzoic acid, ferulic acid, and sinapic acid.Results of the present study indicated that FWE (0.5 ml/mouse for 10 successive days) exhibited a significant reduction (P<0.05) in worm burden.A scan electron microscope examination of male worms showed tegument corrosions with reduced spines density and abnormal spine tubular shape.Treatments of infected mice with FEW or PZQ significantly reduced hepatic and intestinal total egg count, while showed a significant increase in dead egg percentages and reduced mature egg count in oogram pattern as compared to infected control mice.Hepatic granuloma diameters in FWE treated infected mice were significantly reduced when compared to the infected control group.FEW also reduced the lymphocytic infiltration around the granulomatous tissue.It can be concluded that FWE exhibited anti-schistosomal and anti-inflammatory effects on infected mice.