Quantum dot nanobead immunochromatographic assay based on bispecific monoclonal antibody for the simultaneous detection of aflatoxin B-1 and amantadine

A bispecific monoclonal antibody (BsMAb) that can simultaneously recognize aflatoxin B-1 (AFB(1)) and amantadine (AMD) was prepared. Quantum dot nanobead immunochromatographic assay (QB-ICA) based on the BsMAb was developed for the simultaneous detection of AFB(1) and AMD in four feed samples (suckling pig feed, piglet feed, sow feed, and compound feed for laying ducks). Under optimal experimental conditions, the LOD values of QB-ICA for AFB(1) in four feed samples were 0.188, 0.221, 0.233, and 0.306 mu g/kg, respectively. The LOD values of QB-ICA for AMD in the same feed samples were 0.125, 0.187, 0.076, and 0.191 mu g/kg, respectively. The recovery rates of QB-ICA for AFB(1) and AMD ranged from 91.55% to 150.87% and from 91.62% to 138.42%, respectively. The variation coefficients were all less than 15%. The proposed QB-ICA had high reliability and specificity for the simultaneous detection of AFB(1) and AMD, providing a platform for simultaneous detection of hazardous substances.