Tier-based approach to establish a culture of biosafety at a medical microbiology research laboratory in Egypt

Microbiology Research Laboratory (MRL) is a biosafety level-2 (BSL-2) research laboratory located at the main campus of Faculty of Medicine, Ain Shams University (ASU) in Cairo. With the objective of strengthening the departmental capacities of biosafety, a series of activities were carried out between October 2019, and January 2020 to raise awareness, along with instilling standard biosafety practices and procedures among laboratory staff including non-health professions. MRL staff were categorized according to their biosafety knowledge into three tiers: tier (1): with zero to minimal knowledge, tier (2): with basic knowledge, tier (3): with satisfactory knowledge. Tier based activities were designed to align with their job responsibilities. Results: 44 selected laboratory staff were trained on biosafety practices: 12 from tier (1), 19 from tier (2) and 13 constituted tier (3). Through regular follow-ups, the impact of the implemnted training plan was reflected on the practices and knowledge of all laboratory staff. Knowledge among health professions has increased by 60%. Furthermore, 6 staff members have granted a biosafety certification by International Federation of Biosafety Association (IFBSA). Conclusion: establishing a culture of biosafety within microbiology research laboratories is integral to safe research practices. Together with developing local and national biosafety regulations and policies will ensure research advancement without compromising public health or environmental safety.