Detection of SARS-CoV-2 VOC-Omicron using commercial sample-to-answer real-time RT-PCR platforms and melting curve-based SNP assays
Journal of Clinical Virology Plus(2022)
摘要
•Detection performances of sample-to-answer platforms were not impacted by Omicron.•TIB MOLBIOL SNP assays identified spike S371L/S373P & E484A mutations of Omicron.•Unreported melting peak was observed in Omicron variant by TIB MOLBIOL E484K assay.•N501Y dropout was observed in Omicron variant by TIB MOLBIOL N501Y assay.
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关键词
COVID-19,SARS-CoV-2,Coronavirus,Polymerase Chain Reaction,Limit of Detection,Single Nucleotide Polymorphism
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